Volatile Organic Compounds from Rice Rhizosphere Bacteria Inhibit Growth of the Pathogen Rhizoctonia solani

Rice sheath blight, a fungal disease caused by Rhizoctonia solani, seriously threatens rice production. Some of the volatile organic compounds (VOCs) produced microbes are inhibitory to growth plant pathogen, and hence may have potential as environmentally friendly antifungal substances. However, information on effect VOCs released rhizosphere bacteria R. solani is scarce. In this study, from capable inhibiting via releasing were screened using double Petri dish assay. Headspace solid phase microextraction gas chromatography mass spectrometry (GC/MS) used identify quantify VOCs. The contributions inhibition estimated constructing random forest model, verified pure compounds. Nine strains (i.e., Pseudomonas sp. No. 3, Enterobacter 26, 34, 35, Ralstonia 50, Bacillus 62, Arthrobacter 146, Brevibacillus 2–18, Paenisporosarcina 2–60) showed various ranged 7.84% 100%, with 50 completely solani. Five benzoic acid ethyl ester, 3-methyl-butanoic acid, 2-ethyl-1-hexanol, 3-methyl-1-butanol, 6-methyl-5-hepten-2-one) identified model confirmed be toxic when applied chemical compound. particular, 2-ethyl-1-hexanol lethal summary, (Ralstonia 50) (benzoic 2-ethyl-1-hexanol) new resources for biological control blight.